Immunomagnetic Separation Combined with Polymerase Chain Reaction for the Detection of Alicyclobacillus acidoterrestris in Apple Juice

Immunomagnetic Separation Combined with Polymerase Chain Reaction for the Detection of Alicyclobacillus acidoterrestris in Apple Juice

A combination of immunomagnetic separation (IMS) and polymerase chain reaction (PCR) was used to detect Alicyclobacillus acidoterrestris (A. acidoterrestris) in apple juice. The optimum technological parameters of the IMS system were investigated. The results indicated that the immunocapture reactions could be finished in 60 min and the quantity of IMPs used for IMS was 2.5 mg/mL. Then the comb...

Efficacy of microwave against alicyclobacillus acidoterrestris in apple Juice

Apple juice inoculated with alicyclobacillus acidoterrestris was treated with microwave at different power and time, and the efficacy of microwave against alicyclobacillus acidoterrestris was investigated. The results showed that alicyclobacillus acidoterrestris quantity decreased with microwave enhancement or time extension. When juice was treated with microwave at higher than 720 W and for lo...

New methods for inactivation of Alicyclobacillus acidoterrestris spores in apple juice concentrate

Evaluation of Immunomagnetic Separation for the Detection of Salmonella in Surface Waters by Polymerase Chain Reaction

Salmonella spp. is associated with fecal pollution and capable of surviving for long periods in aquatic environments. Instead of the traditional, time-consuming biochemical detection, polymerase chain reaction (PCR) allows rapid identification of Salmonella directly concentrated from water samples. However, prevalence of Salmonella may be underestimated because of the vulnerability of PCR to va...

COMPARISON OF CULTURE WITH POLYMERASE CHAIN REACTION FOR DETECTION OF UREAPLASMA UREALYTICUM IN ENDOCERVICAL SPECIMENS

Background: Ureaplasma urealyticum is known as a sexually transmitted agent, causing mainly urethritis, pelvic int1ammatory disease, spontaneous abortion, pyelonephritis, infertility, stillbirth, low birth weight neonatal meningititis, and neonatal pneumonia. U. urealyticum infections not only jeopardize fertility but also pose a risk for infertility treatment and resulting pregnancies. Dia...